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The question of the subject is how to tell the enzyme the relevant information about the target gene. Many respondents have talked about it for a long time. The crispr system is the acquired (active) immune system of bacteria. Its capture of foreign DNA is based on the pam sequence on the foreign DNA. For foreign DNA with a pam sequence, the cas1 and cas2 proteins in the crispr system will warmly welcome it and leave a sequence around it as its "identity document (wanted order). Cas12a (also known as cpf1) is a type of endonuclease mediated by a single crRNA, which can specifically recognize and cut double-stranded DNA (dsDNA) targets with PAM (protospacer adjacent motif). The PAM sequences recognized by cas12a proteins from different sources may be different. The Cas12a family all recognize T-rich PAM sequences. Pam (pulse amplitude modulation) is a basic digital modulation technology that converts analog signals into digital signals. In matlab, you can use functions in the signal processing toolbox and communication toolbox to implement the simulation of the sampling theorem and pam.
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